Coding

Part:BBa_K3402034

Designed by: Yibo Shi   Group: iGEM20_Jiangnan_China   (2020-10-21)


Rec

Rec will express serine recombinant enzyme.

Usage and Biology

According to the β-Rec/six self-excising system, serine recombinant enzyme will recognize two adjacent six sites in the same DNA strand and specifically cut off the fragments between two adjacent six sequences.
In this study, we use this system to recycle hygromycin resistance gene(hph BBa_K3402012).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 256
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None